Works are underway to remove 92 unstable trees on the western side of Melba Highway between Maroondah Highway and Rudducks Lane.
An assessment from an arborist found that the cypress trees are diseased with Cypress Canker, a fungal disease that has compromised the trees.
VicRoads said they are working closely with Yarra Ranges Council on a replanting strategy to improve the amenity of the area.
Works started on Monday 15 June and are expected to be completed by Tuesday 30 June. The works will take place from Monday to Friday from 7am and motorists are advised to plan ahead and allow for extra time on their journey.
During the works there will be a lane closure in place under traffic management to ensure crews can continue moving in both directions, reduced speed limits to ensure safety of working crews and road users and some levels of noise throughout the removals.