A 41-year-old Chum Creek woman was arrested at the Coldstream Covid-19 vehicle checkpoint on 4 August.
Police said the woman failed to complete an oral fluid test (OFT) and assaulted a police officer at the checkpoint about 8.45am.
She was taken into custody to complete the OFT and is expected to be charged on summons with assault police and drug driving related offences.
Police said the officer sustained a minor graze as a result of the incident.
It came as Victoria Police issued 155 fines for Covid-19 restriction breaches on 5 August, over a 24 hour period.
Of those fines, 36 failed to wear a face covering when leaving the home for essential reasons, five were issued at vehicle checkpoints and 25 were for curfew breaches.
