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Yarra Ranges Highway Patrol officers are investigating the circumstances surrounding a fatal crash in Seville on Sunday 2 March.
It is understood a motorcycle left the roadway and crashed into a tree near the intersection of Parslows and Macclesfield roads just after 11am.
Emergency services worked on the rider, who is yet to formally identified, but he could not be revived.
Anyone who witnessed the crash or with dashcam footage is urged to contact Crime Stoppers on 1800 333 000 or submit a confidential report online at crimestoppersvic.com.au
For the current provisional lives lost tally please see police.vic.gov.au/road-safety