Five poplar trees on River Street in Healesville will be removed this week.
A Yarra Ranges Council spokesperson said the trees, located close to a boundary fence at Healesville railway station pose a risk of falling and damaging infrastructure, including a nearby water tower.
The tree removal is expected to take one day and traffic management will be in place during the works.
The trees are part of the ongoing River Street Tree Replacement Program that has seen trees replaced over the past two years with specially-grown oaks chosen to eventually replicate the iconic River Street vista.